Effectiveness of Collagen β-glucan Composite for Wound Healing Treatment

Document Type : Research Article (Original Research)

Authors

1 faculty of science, chemical department, pre master degree of analytical chemistry

2 Medical Biotechnology Department, Institute of Genetic Engineering and Biotechnology, City of Scientific Research and Technological Applications, Alexandria, Egypt, Pharmaceutical and Fermentation Industry Development Centre, City of Scientific Research

3 Analytical Chemistry Department, Faculty of Science, Mansoura University, Egypt

Abstract

Fish skin-derived collagen and Saccharomyces cerevisiae-derived beta-glucan were extracted for potential use in wound healing applications, with a comprehensive investigation into their physicochemical properties such as molecular weight, solubility, and heat stability. Fourier transform infrared spectroscopy (FTIR) was utilized to examine the chemical bonds of fish collagen and compare its resemblance to type 1 collagen. A mouse model was employed to assess the wound-healing impact of β-glucan. The findings revealed that employing a weak acid (CH3COOH) in combination with a strong base (NaOH) for extraction resulted in high beta-glucan content and an advantageous ratio of polysaccharides, proteins, and lipids. Additionally, rats were utilized to evaluate the healing effects of the chosen β-glucan. The animal model demonstrated that the combination of collagen and β glucan exhibited superior efficacy in wound healing, leading to accelerated wound closure. Based on these results, the isolated fish collagen shows promise for potential utilization in wound treatment.

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