Guide for Authors

Before submission guidelines make sure that the following items are present in the manuscript: The corresponding author’s name with the full contact details are included such as E-mail address, postal address, and contact phone number if possible. Upload all the necessary files (manuscript, keywords, all figures with relevant captions, and all tables. Where applicable highlights files and supplementary files must be uploaded. Also, the manuscript has been well written in English, spelled checked and grammar checked and all references in the Reference List are cited correctly in the text. A competing interest statement must be provided, even if the authors have no competing interest to declare. Whenever it is appropriate Referees suggestions and contact details must be provided based on journal requirements.

Instructions for Authors:

Manuscript Covering Letter. Corresponding authors must submit a cover letter that includes a valid official email (institutional email) address for each author. If this is not possible, the cover letter must confirm that all authors agree to this submission. The corresponding authors must suggest several potential reviewers from different countries and different institutions. Recommending reviewers only from your home country or your institution is likely to result in paper rejection. Please make sure that you provide complete accurate contact information for the suggested reviewers.

Manuscript submission. The submitted manuscript has not been published before and is not under consideration for publication anywhere else and its submission has been approved by all co-authors.  Including tables, figures or text passages that have already been published require permission from the copyright owner and to include evidence that such permission has been approved when submitting the manuscript. The submission process is an Online process, and the corresponding authors must upload all the manuscript files following the instructions on the editorial manager on the screen. Ensure that all relevant editable source files are submitted correctly. Failing to submit these files results in incomplete submission and the submitted manuscript will be sent back to the authors. The manuscript text must be in a common word processing format such as docx.

Title page.

  • The title page must contain the following information.
  • Title. Short, concise, and informative title.

Authors information. All author's names, affiliations, city (state), country and if available, the 16-digit ORCID of the authors. Active e-mail address of the corresponding author.

Example of the Title page:

Recombinant Escherichia coli type-II L-asparaginase with unique L-glutaminase activity: cloning, expression, and biochemical characterization

Rawan Hussiena, Nihal Alyb, Nefertiti El-Nikhelya, Hisham Nematallac, Mohamed Elkewedid, Hesham Saeed a*

a Department of Biotechnology, Institute of Graduate Studies and Research, Alexandria University, Alexandria, Egypt.

b Botany and Microbiology Department, Faculty of Science, Alexandria University, Alexandria, Egypt.

c Department of Pharmacology and Toxicology, Faculty of Pharmacy, Damanhur University, Damanhur, Egypt.

d Department of Medical Laboratory Technology, Faculty of Applied Health Sciences Technology, Pharos University in Alexandria, Alexandria, Egypt.

*Author to whom correspondence should be addressed; E-Mail: manalshalaby2870@yahoo.com, mshalaby@srtacity.sci.eg

Tel.:01023035570    

Abstract. Please provide an abstract of 250-300 words. The abstract should not contain any undefined abbreviations or unspecified references.

Keywords. A maximum of 6 keywords should be provided which can be used for indexing purposes.

Manuscript structure. When submitting the manuscript Online make sure that the following sections are included separately: Abstract, Keywords, Introduction, Materials and Methods, References, Results, Discussion (you can combine Results and Discussion together), Acknowledgements (if required), References, Figure legends (Figure captions), and Tables. Figures and Tables should be supplied as separate files.

Text.

Manuscript should be submitted in Word format. Use a normal, plain font such as 12-point Times New Roman for text and use italics for emphasis. Please use the automatic page numbering function to number the pages and do not use field function. Save your manuscript file in docx format (Word 2007 or higher).  

Headings. Use the decimal system of headings with no more than three levels.

Abbreviations. Abbreviations should be defined at first mention and used consistently after.

Footnotes. Additional information can be given using footnotes which may include citation of a reference in the references list, but they should never include the bibliographic details of the reference. Footnotes to the tables should be indicated by superscript (lowercase or asterisks for significance values and other statistical data).

Introduction. Describe the topic's mechanisms, history, and relationship to a problem. State the work's objectives and provide adequate background information while avoiding a detailed literature survey or a summary of the results. The authors should make efforts to define the significance of the work and the rationale for its publication. Any background discussion should be brief and limited to relevant information. The introduction section should not exceed 3 pages.

Materials and Methods. The Materials and Methods section should provide all the details needed for experiment reproducibility, especially for the new protocols. Well-established protocols can be referenced. Details of large protocol documents may be uploaded as supporting material in a separate file.

Results and Discussion. The Results section should be written in past tense, and, optional, it could be combined with the discussion in the same section “Results and discussion”. It should provide all the details of all the experiments, including information on the number of replicates (if relevant to ensure replicability). There is no specific word limit for this section, but please avoid unnecessary details that distract the focus of the article. The section could be divided into subsections, each with a subheading for each experiment. The Results section should be written in the past tense. The Discussion should indicate the relevant previous conclusions and findings and compare them with the manuscript’s findings. Moreover, it should interpret the work precisely giving some explanation supported with previous knowledge.

Conclusion. To conclude and illustrate how the current findings of the proposed manuscript affect the existing assumptions in a certain field. Should be precise and illustrative in up to 200 words.

Acknowledgment. Acknowledgment of grants, funds, people, etc. should be placed after the Word file and before the references. The names of funding organizations should be written in full detail.

Scientific style. Internationally accepted symbols and signs must be used for units (e.g., mL, µL, µM, etc.). Genus and species names should be in italics. For drugs and pesticides, generic names must be used and are preferred.

Ethical Guidelines for Future Perspectives of Medical, Pharmaceutical and Environmental Biotechnology

Future Perspectives of Medical, Pharmaceutical and Environmental Biotechnology is dedicated to promoting ethical research and publication practices and adheres to a set of principles that safeguard the integrity of scientific knowledge. Our commitment to ethical publishing is grounded in the highest standards and values of academia. We expect all individuals involved in the publication process to uphold these ethical principles:

Originality and Avoidance of Plagiarism: Authors must ensure that their submitted work is entirely original and has not been published elsewhere. They should provide proper citations and references when referring to prior work. Any form of plagiarism, including self-plagiarism, is unacceptable.

Authorship Criteria: Authorship should be limited to individuals who have made substantial contributions to the research and the manuscript's preparation. All authors should be listed and should have consented to the final version of the manuscript. Any changes to authorship should be collectively agreed upon.

Data Transparency and Reproducibility: Authors are responsible for providing accurate and complete data associated with their research. Data should be made openly available, and authors should be ready to grant access to raw data upon request.

Conflict of Interest Disclosure: Authors must disclose any financial or non-financial conflicts of interest that could potentially affect the research or its interpretation. These disclosures should be clearly stated in the manuscript.

Ethical Approvals: Research involving human subjects or animals should have obtained the requisite ethical approvals. Authors should furnish details of these approvals in the manuscript.

Artificial Intelligence Generated Content (AIGC) tools, such as ChatGPT and others utilizing extensive language models (LLMs), cannot independently initiate original research without human author guidance. They also cannot assume responsibility for a published work or research design, a fundamental requirement typically associated with authorship (as discussed in the preceding section). Additionally, AIGC tools lack legal standing and the capability to assert or transfer copyright ownership. Consequently, in alignment with the position statement on AI tools by the Committee on Publication Ethics (COPE), these tools cannot fulfill the role of an author of an article and should not be credited as such. In cases where an author has utilized such tools in the development of any portion of a manuscript, it is imperative to describe their use transparently and comprehensively in either the Methods or Acknowledgements section. The author bears full responsibility for the accuracy of any information generated by the tool and must properly reference any underlying work upon which that information relies. It is important to note that tools employed for enhancing spelling, grammar, and general editing are not encompassed by these guidelines. The ultimate determination of whether the use of an AIGC tool is suitable or permissible in the context of a submitted manuscript or a published article rests with the journal's editor or the relevant party overseeing the publication's editorial policies.

References. References citations in the text should be identified in numbers in square brackets. For example:

  • Unlike normal cells, leukemia cells are unable to synthesize L-Asn because of low expression, or complete absence of the asparagine synthetase (ASNS) gene. [5]
  • Thus, administration of L-ASNase to ALL patients results in the depletion and scavenging of the L-Asn supply to these cells, leading ultimately to cell death. [6]
  • Reference List. The references list must include cited works in the text that have been published or accepted for publication. References should be numbered consecutively and if available, please include the DOIs as full DOI links in the reference list. Example of DOI: https://doi.org/10.1080/10826068.2021.1983831.

Example of journal article reference:

[13] Reinert, R. B.; Oberle, L. M.; Wek, S. A.; Bunpo, P.; Wang, X. P.; Mileva, I.; Goodwin, L. O.; Aldrich, C. J.; Durden, D. L.; McNurlan, M. A.; et al. Role of Glutamine Depletion in Directing Tissue-Specific Nutrient Stress Responses to L-asparaginase. J. Biol. Chem. 2006, 281, 31222–31233. DOI: 10.1074/ jbc.M604511200.

Book reference:

  • Sambrook, H. Molecular Cloning: A Laboratory Manual, 2nd ed.; Cold Spring Harbor Laboratory Press: New York; 1989.
  • Book chapter. G.R. Mettam, L.B. Adams, How to prepare an electronic version of your article, in: B.S. Jones, R.Z. Smith (Eds.), Introduction to the Electronic Age, E-Publishing Inc., New York, 2009, pp. 281–304.
  • Website. Cancer Research UK, Cancer statistics reports for the UK. http://www.cancerresearchuk.org/aboutcancer/statistics/cancerstatsreport/, 2003 (accessed 13 March 2003).
  • Dataset. M. Oguro, S. Imahiro, S. Saito, T. Nakashizuka, Mortality data for Japanese oak wilt disease and surrounding forest compositions, Mendeley Data, v1, 2015. https://doi.org/10.17632/ xwj98nb39r.1.
  • Software. A.C. Wallace, R. A. Laskowski, J.M. Thornton, LIGPLOT: a program to generate schematic diagrams of protein-ligand interactions, Protein engineering, 8(1995)127–134. https://doi.org/10.1093/protein/8.2.127.

Supporting information. Authors can submit auxiliary supporting files as raw data, large dataset results and multimedia files, if it would support the understanding and reproducibility of their work. All the Supporting Information will be subject to peer review. These files will be published exactly as provided. Please list supporting information captions at the end of the manuscript file.

Tables. Upload tables file as DOC, or PTTX separate file or include tables within the manuscript.

Cite tables in ascending numeric order upon first appearance in the manuscript file. If the tables are included in the manuscript, please place each table after the paragraph in which it is first cited.

Do not insert your tables as images. Tables require a label (e.g., “Table 1”) and brief descriptive title to be assigned above the table. Place legends, and other text below the table.

After acceptance. Online proof correction to ensure a fast publication process of the article, we kindly ask authors to provide us with their proof corrections within four days. Corresponding authors will receive an e-mail with a link to our online proofing system, allowing annotation and correction of proofs online. The environment is like MS Word: in addition to editing text, you can also comment on figures/tables and answer questions from the Copy Editor. Web-based proofing provides a faster and less error-prone process by allowing you to directly type your corrections, eliminating the potential introduction of errors. If preferred, you can still choose to annotate and upload your edits on the PDF version. All instructions for proof will be given in the e-mail we send to authors, including alternative methods to the online version and PDF. We will do everything possible to get your article published quickly and accurately. Please use this proof only for checking the typesetting, editing, completeness and correctness of the text, tables, and figures. Significant changes to the article as accepted for publication will only be considered at this stage with permission from the Editor. It is important to ensure that all corrections are sent back to us in one communication. Please check carefully before replying, as inclusion of any subsequent corrections cannot be guaranteed. Proofreading is solely your responsibility. Offprints The corresponding author will, at no cost, receive a customized Share Link providing 50 days free access to the final published version of the article on ScienceDirect. The Share Link can be used for sharing the article via any communication channel, including email and social media. For an extra charge, paper offprints can be ordered via the offprint order form which is sent once the article is accepted for publication.